Platform Engineering : Corynebacterium glutamicum with reduced 1 pyruvate dehydrogenase complex activity for improved production of L - 2 lysine , L - valine , and 2 - ketoisovalerate 3 4
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Platform Engineering: Corynebacterium glutamicum with reduced 1 pyruvate dehydrogenase complex activity for improved production of L2 lysine, L-valine, and 2-ketoisovalerate 3 4 Jens Buchholz, Andreas Schwentner, Britta Brunnenkan, Christina Gabris, 5 Simon Grimm, Robert Gerstmeir, Ralf Takors, Bernhard J. Eikmanns, and 6 Bastian Blombach 7 8 Institute of Biochemical Engineering, University of Stuttgart, D-70569 Stuttgart, 9 Institute of Microbiology and Biotechnology, University of Ulm, D-89069 Ulm, and 10 Evonik Degussa GmbH, Kantstraße 2, D-33790 Künsebeck, 11 12 13 * Corresponding author. Mailing address: Institute of Biochemical Engineering, 14 University of Stuttgart, 70569 Stuttgart, Germany. Phone: 49 (0)711 685 64549. Fax: 49 15 (0)711 685 65164. E-mail: [email protected] 16 17 Running title 18 Platform engineering with C. glutamicum 19 20
منابع مشابه
Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovalerate.
Exchange of the native Corynebacterium glutamicum promoter of the aceE gene, encoding the E1p subunit of the pyruvate dehydrogenase complex (PDHC), with mutated dapA promoter variants led to a series of C. glutamicum strains with gradually reduced growth rates and PDHC activities. Upon overexpression of the l-valine biosynthetic genes ilvBNCE, all strains produced l-valine. Among these strains,...
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Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE sh...
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تاریخ انتشار 2013